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  Actomyosin-assisted pulling of lipid nanotubes from lipid vesicles and cells

Jahnke, K., Maurer, S. J., Weber, C., Hernandez Bücher, J. E., Schoenit, A., D´Este, E., et al. (2022). Actomyosin-assisted pulling of lipid nanotubes from lipid vesicles and cells. Nano Letters, 22(3), 1145-1150. doi:10.1021/acs.nanolett.1c04254.

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 Creators:
Jahnke, Kevin1, Author           
Maurer, Stefan J.1, Author           
Weber, Cornelia1, Author           
Hernandez Bücher, Jochen Estebano1, Author           
Schoenit, Andreas1, Author           
D´Este, Elisa2, Author           
Cavalcanti-Adam, Elisabetta Ada1, Author           
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

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Free keywords: Lipid nanotubes, lipid tether pulling, motility assay, giant unilamellar vesicle, membrane-to-cortex attachment, actin, heavy mero-myosin
 Abstract: Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In
particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.

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Language(s): eng - English
 Dates: 2022-01-232021-11-042022-01-282022
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.1c04254
 Degree: -

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
 Creator(s):
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 1145 - 1150 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403
 
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