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  Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry

Seedorf, H., Kahnt, J., Pierik, A. J., & Thauer, R. K. (2005). Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry. The FEBS Journal, 272(20), 5337-5342. doi:10.1111/j.1742-4658.2005.04931.x.

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Seedorf, H.1, Author           
Kahnt, J.1, Author           
Pierik, A. J., Author
Thauer, R. K.1, Author                 
Affiliations:
1Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              

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Free keywords: coenzyme F-420; 5-deazaflavins; F420H2 oxidase; methanogenic Archaea; stereospecificity
 Abstract: Coenzyme F420 is a 5-deazaflavin. Upon reduction, 1,5 dihydro-coenzyme F420 is formed with a prochiral centre at C5. All the coenzyme F420-dependent enzymes investigated to date have been shown to be Si-face stereospecific with respect to C5 of the deazaflavin, despite most F420-dependent enzymes being unrelated phylogenetically. In this study, we report that the recently discovered F420H2 oxidase from methanogenic Archaea is also Si-face stereospecific. The enzyme was found to catalyse the oxidation of (5S)-[5-2H1]F420H2 with O2 to [5-1H]F420 rather than to [5-2H]F420 as determined by MALDI-TOF MS. (5S)-[5-2H1]F420H2 was generated by stereospecific enzymatic reduction of F420 with (14a-2H2)-[14a-2H2] methylenetetrahydromethanopterin.

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Language(s): eng - English
 Dates: 2005-10-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 261693
ISI: 000232438800021
DOI: 10.1111/j.1742-4658.2005.04931.x
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Title: The FEBS Journal
  Other : The Federation of European Biochemical Societies Journal
Source Genre: Journal
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Publ. Info: Wiley-Blackwell
Pages: - Volume / Issue: 272 (20) Sequence Number: - Start / End Page: 5337 - 5342 Identifier: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485
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