Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry

Seedorf, H., Kahnt, J., Pierik, A. J., & Thauer, R. K. (2005). Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry. The FEBS Journal, 272(20), 5337-5342. doi:10.1111/j.1742-4658.2005.04931.x.

Item is

Externe Referenzen

einblenden:
ausblenden:
Beschreibung:
-
OA-Status:
Hybrid

Urheber

einblenden:
ausblenden:
 Urheber:
Seedorf, H.1, Autor           
Kahnt, J.1, Autor           
Pierik, A. J., Autor
Thauer, R. K.1, Autor                 
Affiliations:
1Department of Biochemistry, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society, ou_3266311              

Inhalt

einblenden:
ausblenden:
Schlagwörter: coenzyme F-420; 5-deazaflavins; F420H2 oxidase; methanogenic Archaea; stereospecificity
 Zusammenfassung: Coenzyme F420 is a 5-deazaflavin. Upon reduction, 1,5 dihydro-coenzyme F420 is formed with a prochiral centre at C5. All the coenzyme F420-dependent enzymes investigated to date have been shown to be Si-face stereospecific with respect to C5 of the deazaflavin, despite most F420-dependent enzymes being unrelated phylogenetically. In this study, we report that the recently discovered F420H2 oxidase from methanogenic Archaea is also Si-face stereospecific. The enzyme was found to catalyse the oxidation of (5S)-[5-2H1]F420H2 with O2 to [5-1H]F420 rather than to [5-2H]F420 as determined by MALDI-TOF MS. (5S)-[5-2H1]F420H2 was generated by stereospecific enzymatic reduction of F420 with (14a-2H2)-[14a-2H2] methylenetetrahydromethanopterin.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2005-10-01
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 261693
ISI: 000232438800021
DOI: 10.1111/j.1742-4658.2005.04931.x
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: The FEBS Journal
  Andere : The Federation of European Biochemical Societies Journal
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Wiley-Blackwell
Seiten: - Band / Heft: 272 (20) Artikelnummer: - Start- / Endseite: 5337 - 5342 Identifikator: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485
OSZAR »